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Cell SystemsResearch article · 2025

Mapping regulators of stress resilience with pooled CRISPR screening

Methods excerpt · pooled screen + scRNA-seq

Evidence source

Methods

A genome-scale CRISPR guide library was introduced across three biological replicates. Cell states were profiled by single-cell RNA sequencing and analysed in a reproducible pipeline.

John

Experimental design 8 skills · 12 papers

From this paper

Designed the CRISPR screen

Source Pooled CRISPR study 3 colleague confirmations
Also on profile
  • Flow cytometry
  • Assay design
  • +5 skills · 11 papers

Mary

Data generation 6 skills · 9 papers

From this paper

Ran single-cell RNA sequencing

Source Pooled CRISPR study 2 colleague confirmations
Also on profile
  • RNA extraction
  • Cell culture
  • +3 skills · 8 papers

Eve

Software & analysis 11 skills · 15 papers

From this paper

Built the analysis pipeline

Source Pooled CRISPR study 1 colleague confirmation
Also on profile
  • Python
  • Data visualisation
  • +8 skills · 14 papers
Linked source: Pooled CRISPR study, 2025

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    Mapping regulators of stress resilience with pooled CRISPR screening

    Cell Systems · 2025 · John, Mary, Eve, et al.

    Skill and research role

    John · Designed the CRISPR screen

    Linked source: Pooled CRISPR study

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    Skill and research role

    John · Designed the CRISPR screen

    Linked source: Pooled CRISPR study

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    • A. BrownConfirmed
    • L. ChenConfirmed
    • R. KumarConfirmed

    3 colleague confirmations

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    John · Designed the CRISPR screen

    Linked source: Pooled CRISPR study

    Confirmed by colleagues

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    Pooled screening study design

    Matched evidence: CRISPR screen design

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